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Cell3iMager Estier

Coming soon.

Cell3iMager Neo

Powerful 3D software handles routine and complex workflows Fast, high-resolution bright field optics and image capture are only half of the Cell3iMager package. Scanning more plates faster means more data to analyze, and the Cell3iMager’s intelligent software provides the power needed to keep up. The software reports key spheroid growth and morphological parameters, including spheroid number, size, shape, and growth kinetics over time. Basic scanning setup parameters are included, and intelligent object identification settings can be customized and saved for repeated use to ensure accuracy and reproducibility within and between experiments. Growth curves and histograms are generated and can be copy/pasted directly, or raw data can be exported as CSV files for import into statistical and graphing software. Supports basic (single-time point) and time-course dose-response analysis, as well as a multi-planar (focus bracketing) scanning option to use with hydrogel-embedded spheroids 30+ adjustable parameters for optimizing object identification, live/dead discrimination (e.g., bubbles, fibers, debris), and compensate for edge of well shadows and distortion as well as reduction of background noise Identify and measure microtissues in single spheroid per well or multiple spheroid per well formats Software & Accessories The Cell3iMager spheroid analysis software allows processing of high magnification images of your 3D cell culture, including single or multiple spheroids, neurospheres, organoids, or embryoid bodies.

Cell3iMager Duos

Powerful image processing and analysis software

The companion analysis software of duos allows faster image capture, measurement and data analysis, and is compatible with all major cell culture platforms. The powerful automated measurement and analysis software eliminates cumbersome manual morphological measurements and improves data reproducibility. The software reports key cellular growth and morphological parameters overtime such as:

∙ Accurate cell counting (total, live & dead cells)
∙ Colony segmentation
∙ Cell proliferation (including antibody forming cells)
∙ Spheroid / organoid area, diameter, circularity, optical density and pseudo volume

Basic scanning setup parameters are included, and intelligent object identification settings can be customized and saved for repeated use to ensure accuracy and reproducibility within and between experiments.

An automatic cell morphological classification (ACMC) option provides ‘intelligent’ automatic classification of live and dead spheroids, using logic derived from a user-defined reference set of live/dead spheroids.

Growth curves and histograms are generated and can be copy/pasted directly, or raw data can be exported as CSV files for import into statistical and graphing software and stitched images can be saved anywhere you want. In short, this is an imager that record the entire story of your cells.

Focus bracketing

Duos provide a unique focus bracketing option (similar to ‘Z’ stacking) which enables high quality analysis of spheroids in hanging droplet assays or embedded in hydrogel systems, which otherwise can’t be adequately captured.

Focus bracketing acquires images over multiple focal planes/slices to provide a multi layered image. With duos, up to 50 slices can be obtained to capture all important details of the spheroids or organoids. Duos composite function offer great flexibility to combine/quantify mass of cells spread in the ‘Z’-direction into a single information loaded image.

Cell3iMager Estier specifications
Well plates 6 to 384 wells, flat and U-shape bottom types and 35 mm dish
Microscope slide, 25cm2 flask (only for Cell3iMager neo)
Plate number 4
Scan speed 54 seconds per plate (2400 dpi high speed mode)
Automated measuring time 20 seconds per plate (2400 dpi high speed mode)
Scan resolutions 200dpi, 300dpi, 600dpi, 1200dpi, 2400dpi, 4800dpi, 9600dpi
Light mode/optical system White LEDs/transmission illumination
Measuring Number, area, estimated volume, contour, optical density, circularity and edge sharpness
Output format – Captured image display and numerical data confirmation using base software

– Granular distribution and proliferation trend chart confirmation

– Output of measured data in CSV file

Environment Recomended: Temperature 18 to 28 oC(64 to 82oF)
Power requirements Single phase 100 – 240V 0.12 kw 0.9A
Dimensions (W x D x H) 492 x 755 x 294 mm
Weight Approx. 40 kg
Cell3iMager Neo specifications
Well plates 6 to 384 wells, flat and U-shape bottom types and 35 mm dish

Microscope slide, 25cm2 flask (only for Cell3iMager neo)

Plate number 1
Scan speed 50 seconds per plate (2400 dpi high speed mode)
Automated measuring time 20 seconds per plate (2400 dpi high speed mode)
Scan resolutions 200dpi, 300dpi, 600dpi, 1200dpi, 2400dpi, 4800dpi, 9600dpi
Light mode/optical system White LEDs/transmission illumination
Measuring Number, area, estimated volume, contour, optical density, circularity and edge sharpness
Output format – Captured image display and numerical data confirmation using base software

– Granular distribution and proliferation trend chart confirmation

– Output of measured data in CSV file

Environment Recomended: Temperature 18 to 28 oC(64 to 82oF)
Power requirements Single phase 100 – 240V 0.114 kw 0.85A
Dimensions (W x D x H) 594 x 381 x 294 mm
Weight Approx. 25 kg
Cell3iMager duos features and specifications
Well plates 6,12,24,48,96,384-wells, flat and U-bottom types, 35mm dish
Plate number 01
Scan speed 70 seconds/plate
Scan resolutions 0.8μm, 4μm
Light mode Bright-field & fluorescent
Measure Single cell count, colony segmentation, live-dead cell number, spheroid area, pseudo volume, circularity, diameter, and optical density
Focal plane Auto-focus, manual, multi-plane
Imaging Clarity +++
Budget-Friendly +++

Cell3iMager Estier

Coming soon.

 

Cell3iMager Neo

Q. What does the Cell3iMager do?

A. The Cell3iMager is a high-throughput bright-field scanner with an integrated 3D software which is the only cost effective, non-invasive solution for the kinetic growth profiling of 3D tumor spheroids. It is the fastest bright-field imager capable of scanning a 96-well plate in less than 1 minute.

Q. What cell culture platforms are compatible with the Cell3iMager?

A. The Cell3iMager is compatible with InSphero’s scaffold-free 3D cell culture platforms, including the GravityPLUS™ Hanging Drop System and GravityTRAP™ ULA Plates. The imager is also compatible with a wide range of 3D and traditional culture plates. Please see the accessories section for the most up-to-date list.

Q. How fast is the Cell3iMager and what throughput rates can be achieved?

A. Scanning time depends upon the number of wells being scanned and the desired resolution. Typical scanning and measurement time for 96- and 384-well plates at varying resolutions is shown in the table below. A total number of thirty 384-well plates can be scanned within 1 hr (cc-5000) at 2400 dpi resolution, the resolution recommended for use with InSphero plates. Image resolution is adjustable from 200 dpi, 300 dpi, 600 dpi, 1200 dpi, 2400 dpi, 4800 dpi and 9600 dpi. Scanning times are the same for the cc-3000 and cc-5000 models, although throughput will be greater for the 4-plate cc-5000 due to the multi-plate format which reduces plate handling time.

Typical scanning times

Resolution 96-well 384-well
2400 dpi 54 sec 2 min
4800 dpi 2 min 8 min
9600 dpi 4 min 16 min

Q. Does the Cell3iMager have fluorescence or luminescent detection capabilities?

A. No, neither Cell3iMager unit includes multi-mode functionalities like fluorescence or luminescent detection capabilities. They are bright field scanners with an LED light source designed specifically for rapid bright field image capture and analysis.

Q. Is it possible to take color images?

A. Yes. It is possible to take color images. The software allows to save the scanned image in RGB (red, green, blue) or grayscale.

Q. Can the Cell3iMager analyze multiple plates at a time?

A. It depends. The Cell3iMager (cc-5000) can analyze 4 plates in a single scanning run, giving you the flexibility to scan up to four different plate types at a time if desired. The lower-cost Cell3iMager Neo is capable of scanning just a single-plate in one scanning run.

Q. Can the Cell3iMager be used for 2D cell imaging?

A. Yes. Both Cell3iMager models can be used for bright field imaging of stained 2D cell culture.

Q. Is the Cell3iMager compatible with an automated/robotic loading system?

A. Yes. Both the Cell3iMager Neo (cc-3000) and Cell3iMager cc-5000 are compatible with a plate stacker or robotic loading system. Please contact us for more details about robotic loading system setup.

Q. What are the recommended minimum PC specifications for the Cell3iMager?

A. Cell3iMager software included with each imager is compatible with Windows 7® or Windows 8® professional SPI 64 bit operating systems. The software is NOT compatible with MAC OS. Minimum recommended PC specifications are 3.0 GHz Pentium processor (or compatible Core i5 CPU), 8 GB system memory, 1,280 x 1,024 / 1,600 X 90 / 1,440 X 900 / 1,920 X 1,080 / 1,600 X 1,024 pixel screen resolution, USB 2.0 ports and 400 GB or more HD free space is recommended.

Q. Do you offer on-site demonstrations of the Cell3iMager?

A. Yes, we offer on-site demonstration of the Cell3iMager. A technical expert from SCREEN can visit you on-site to assist with initial instrument setup and software operation. Contact us (screen_lifescience@emis.screen.co.jp) to request an on-site demo.

 

Cell3iMager Duos

Coming soon.

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