Neuronal Differentiation


Differentiation efficiency of β-NGF-treated PC12 was evaluated by using Cell3iMager duos.

Materials and Methods

  • Cell Line: PC12 cells (RIKEN BRC)
  • Medium: DMEM (Nacalai tesque)
  • Plate: 96-well plate flat bottom (Sumitomo Bakelite)
  • Seeding cell density: 500 cells / well
  • Culture days: 0 - 7 days
  • Reagent: β-NGF (Sigma)
  • Imaging methods: High magnification lens (half resolution)

Results and Conclusions

  • You can quantify individually neurites and cell bodies.
  • These figures show that neurite length of NGF-treated cells are higher than control  cells.



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