Suspension cultured cells


Suspension cultured cells were quantified by using Cell3iMager duos.

Materials and Methods

  • Cell Line: Hybridoma 4B2 cells (ATCC)
  • Medium: RPMI (ATCC)
  • Plate: 96-well plate flat bottom (Corning)
  • Seeding cell density: 0 - 10000 cells / well
  • Culture days: 2 days
  • Imaging methods: Bright field, Low magnification lens

Results and Conclusions

  • You can quantify suspension cultured cells easily because stage is fixed during imaging.


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