Cell Proliferation

Proliferation ratio of the 2D/3D cultured cells is quantified by Cell³iMager duos.

Cell Line: HepG2 cells (RIKEN BRC)
Medium: DMEM (Nacalai tesque)
Plate
- 96-well plate flat bottom (Sumitomo Bakelite)
- 96-well plate U bottom (Sumitomo Bakelite)
- 96-well plate flat bottom,
- ultra-low attachment coated (Corning)
Seeding cell density: 1000 cells / well
Culture days: 4 days
Reagent: Hypoxia probe LOX-1(Organogenix)
Imaging methods: Bright field, Low-magnification lens

This quantification can be alternative method to MTT assay and ATP assay. (Correlation with ATP assay has been confirmed.)
It is possible to quantify proliferation ratio of the 2D/3D cultured cells.
Incase of 2D cells, proliferation ratio was quantified by Area.
Incase of 3D cells, proliferation ratio was quantified by Volume.