Thickness of cell sheet


NHDF sheets were imaged by Cell3iMager Estier, and thickness of sheets were calculated from these images.

Materials and Methods

Cell Line

NHDF (PromoCell)


DMEM (Nacalai tesque)


Cell layered culture reagent


Transwell® 24well 0.4μm (Corning)

Seeding cell density

2.15 x 105 cells / well

Culture days

4 days after seeding

Imaging parameters

(Lens) Low magnification les
(Scan Pitch) 5μm
(Scan Area) 3000μm x 3000μm

Results and Conclusions

It is possible to quantify the changing thickness of cell sheets   without damaging cells.



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