NHDF sheets were imaged by Cell3iMager Estier, and thickness of sheets were calculated from these images.
NHDF (PromoCell)
DMEM (Nacalai tesque)
Cell layered culture reagent
Transwell® 24well 0.4μm (Corning)
2.15 x 105 cells / well
4 days after seeding
(Lens) Low magnification les
(Scan Pitch) 5μm
(Scan Area) 3000μm x 3000μm
It is possible to quantify the changing thickness of cell sheets without damaging cells.