Deep Learning | Analysis of spheroids in microwells (EZSPHERE® plate)

Analysis of spheroids in microwells (EZSPHERE® plate)

Analysis of spheroids in microwells (EZSPHERE® plate)

EZSPHERE® | Spheroid | Deep Learning | Microwell

Objective

Spheroids were cultured with EZSPHERE® PLATE and analyzed using Deep Learning plug-in.

Deep Learning | Analysis of spheroids in microwells (EZSPHERE® plate)

Materials and Methods

  • Cell Line: MCF7 (RIKEN BRC)
  • Medium: DMEM (Nacalai tesque)
  • Plate: EZSPHERE® SP MICROPLATE 96 well plate (IWAKI)
  • Seeding Cell density: 1250-40000 cells/well
  • Culture days: 1-4 days
  • Imaging methods: High magnification lens, Manual Focus
  • Analysis: DeepLearning plugin (option
Deep Learning | Analysis of spheroids in microwells (EZSPHERE® plate)

Results and Conclusions

  • Spheroids in microwells can be quantified in a label-free manner.
  • By using the DeepLearning plugin, even complex shaped wells. It is possible to detect only cells.
  • You can compare and examine the size of spheroid within 1 well.
Spheroids in microwells can be quantified in a label-free manner
You can compare and examine the size of spheroid within 1 well.

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