Tumor spheroids - 3D Cell Culture by using Matrigel®

3D Cell Culture by using Matrigel®

3D Cell Culture by using Matrigel®

Spheroid | 3D culture | Gel culture | Label free assay

Objective

Using Cell3iMager duos is effective for quantification of spheroids volume cultured in Matrigel.

Tumor spheroids - 3D Cell Culture by using Matrigel®

Materials and Methods

  • Cell Line: HepG2 cells (RIKEN BRC)
  • Medium: DMEM (Nacalai tesque)
  • Plate: 96-well plate flat bottom (Sumitomo Bakelite)
  • Seeding cell density: 0 - 5000 cells/well
  • Culture days
    • 6 days
    • Imaging methods: Bright field
    • Low magnification lens
    • Multi focus
    • (0.1 mm pitch, 9 shots)
Tumor spheroids - 3D Cell Culture by using Matrigel®

Results and Conclusions

  • The increment of volume SUM depends on cell density. ('Volume' is calculated as; Optical Density x Area = pseudo volume)
  • 3D cultured samples can be quantified by scanning multiple times in the z direction, and synthesizing the images.
  • The quantification Cell3iMager duos can be perform on various culture methods such as ‘in Matrigel’ and ‘on Mtrigel’. culture.
The quantification Cell3iMager duos can be perform on various culture methods such as ‘in Matrigel’ and ‘on Mtrigel’. culture.

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