Robust and versatile imaging technology for a broad spectrum of in vitro cell based assays
Research field and applications
Oncology and regenerative medicine including Neurological and Cardiology applications
2D based high throughput Drug Screening, profiling, efficacy and toxicological assessment for further development, Neurite outgrowth measurement
Cell based assays: Cell counting, clonal selection, proliferation, apoptosis and cell death measurement
3D based assays include quantification and analysis for organoids, tumor spheroids for drug discovery, development, profiling and efficacy measurement, toxicology assessment using 3D hepatotoxicity as an end point measurement
3D neurosphere growth monitoring and neurite outgrowth assessment
High-end model for both 2D and 3D culture analysis
Possible to observe laminar cells such as epithelial cells clearly at the same level as phase-contrast microscope
A high-throughput imager for a variety of applications such as single cell cloning, ESc, iPSc and organoids morphology analysis
Assessing Neurite outgrowth
Function enhancement by plug-in software for Neurite and Deep Learning tool
Automation systems (optional)
Multi-fluorescence imaging / High-throughput
1 minute to scanning and 30 seconds analyzing
Multi-fluorescence imaging and analysis available(Optional)
Cell3iMager Duos 2
Interested in imaging/ quantification of more than 1 biomarker/protein of interest at a time? DUOS 2 can easily do that for you
1. Multi-fluorescence imaging and analysis
Multi-fluorescence imaging and analysis
Bright field and 3 fluorescent colors are automatically and continuously imaged with the simultaneous loading of LED fluorescent filter
Line up of 5 types of fluorescent filters
2. Meniscus-less
Less meniscus and clear imaging even at the peripheral area
Unique hyper-centric and tele-centric optical systems enables a high-resolution imaging of whole well including the marginal area of well
Lens have 2 resolution which has 0.8um/pix and 4um/pix
Equipped with high accurate extraction algorithm even cells soon after seeing
High speed mode provide 60 sec/ 96 well plate and 70 sec/ 384 well plate
3. Deep Learning Plug-in (optional)
Deep Learning to extract and quantify only grown organoids
High confluence and non-uniform images can be accurately extracted and measured using Deep Learning
4. Neurite elongation plug-in (optional)
Cell body area sum and Neurite length
Multi-object analysis, such as neurite elongation, is provided as a plug-in software
5. 3D cellular imaging
Reconstruction and Anylasis of 3D Cultured Cell Images
Equipped with a proprietary lens with a deep depth of field suitable for 3D cultured cell imaging and illumination
Z-stacking imaging and focus composition functions Compatible with F-bottom, V-bottom, U-bottom, various SBS formats and microwell plates as standard
Functional specialty plates such as plates for 3D culture, Corning® Elplasia® (Corning Japan) and EZSPHERE (AGC Techno Glass Co., Ltd.) are also available
6. Automation system
Automation by connecting external devices such as plate stacker and incubator with a plate transfer robot
Automatically captures up to 200 images per day by robotization, streamlining a complicated workflow Specification
Cell3iMager Duos 2 Specifications
DUOS 2 offers wide range of possibilities to perform multiparametric cell based assays
Product name (type)
Cell3iMager duos2 (CC-8300)
Channel
Brightfield / Color Brightfield / Fluorescent
Bright-field light source
White LED strobes
Camera
CMOS 4.2 megapixel color
Optics
Proprietary high-percentic optics (in high speed mode) / Proprietary telecentric optics (in high definition mode)
Resolution
4.0um (in high speed mode) / 0.8um (in high definition mode)
Auto focus
HW: Laser type real time auto focus / SW: Image contrast software auto focus
Image output
24-bit color (8ｰbit×3)
Multicolor
3 fluorescence wavelengths (select from 5 wavelengths)
Fluorescent filter Cube
DAPI, GFP, Cy3, Texas Red, Cy5
Internal temperature
Automatic adjustment to 35°C ±2°C (when device power is on)
Installation Environment
Room temperature 18-28°C, humidity 80% or less (no condensation)
Transport conditions
0-55°C and 80% humidity or less (no condensation) in the package
Culture Vessel
6, 12, 24, 48, 96, 384 well plate (for almost all SBS compliant products ) / 35, 60,100mm dish / glass slides
Power supply
AC100-240V / 250VA
Dimensions & Weight
W677 x D580 x H550 mm / 111 kg
Software
Dedicated Cell3iMager software (included as standard)
Computer
HP Z4 G4 workstation, OS: Windows 10
Would you like additional information?
Cell3iMager Duos 2
A high-throughput, high-resolution imager for multi-fluorescence imaging of 3D and 2D cultured cells.
