Ideal for Cosmetics product testing and evaluation
The relevance of 3D cell culture models in drug efficacy or toxicity studies for various therapeutic areas has increased in recent years. In this context, the organotypic culture of skin biopsies and reproduction of skin tissue in in vitro laboratory setup is being studied in detail for its preclinical and clinical relevance. A sheet like epidermal tissues cultured on insert well are widely used in assays as a skin model. Thickness of the skin sheets is known as one of these phenotypes, and it is an effective feature for capturing morphological changes. In order to measure the thickness, the skin tissue section are commonly prepared by the paraffin embedding method to measure the length from the upper part to the lower part of the tissue. Moreover, this method is invasive, therefore any subsequent assays cannot be performed. Additionally, the evaluation is based on a tissue section locally , hence, it is difficult to capture features such as distribution of tissue thickness across the tissue. Furthermore, embedd ing the tissue with paraffin is difficult to maintain the original shape of the tissue when embossed in paraffin. In order to perform appropriate thickness evaluation, a new method for non invasively observing and quantifying the tissue. OCT tomography (OCT) has become widespread as a three dimensional diagnostic method for the fundus. OCT is a technique for rendering a three dimensional tissue as a tomographic image, and the near infrared light, used by OCT as a light source, is suitable for biological permeability and low in cytotoxicity.
In this study, the OCT imaging device Cell3iMager Estier (SCREEN Holdings Co., Ltd.) was used to acquire 3D images of non invasively stacked cell tissues, and we aimed to establish a n ew method for measuring of tissue thickness distribution. The cell sheet on the insert well was observed using Cell3iMager Estier , and a three dimensional image was obtained. Based on the obtained three dimensional image, cell regions were extracted by imag e processing, and the thickness distribution of the entire sheet was calculated. We report that it is possible to measure non invasively the change of the thickness distribution of the whole cell s heet in vitro.Skin irritation test: SLS (0.3%) exposure to the tissue resulted in changes in the epidermal thickness