3D Cell Culture in Gravity TRAP


Spheroids formed by the method of hanging drop were moved to Gravity TRAPTM ULA plate, and quantified by Cell3iMager neo.

Materials and Methods

  • Cell Line
    • HepG2 cells (RIKEN BRC)
    • NIH3T3 cells (RIKEN BRC)
  • Medium: DMEM (Nacalai tesque)
  • PlateGravityTRAP™ ULA plate (InSphero)
  • Seeding cell density: 500, 1000cells / well
  • Culture days: 5 days after spheroid formation by hanging drop
  • Imaging methods
    • Bright-field, 4800dpi
    • Bracket Focus (stacked)

Results and Conclusions

It was possible to quantify the spheroids formed by hanging drop for growth rate estimation.


Related Product

Would you like additional information?

I agree to the terms and conditions

I agree to the GDPR

Need help finding the right product?
Get in touch
+1 (844) 454-3399