3D Cell Culture in Gravity TRAP

3D Cell Culture in Gravity TRAP

Spheroid | 3D culture | Hanging Drop | Label free assay

Introduction

Spheroids formed by the method of hanging drop were moved to Gravity TRAPTM ULA plate, and quantified by Cell3iMager neo.

Materials and Methods

  • Cell Line
    • HepG2 cells (RIKEN BRC)
    • NIH3T3 cells (RIKEN BRC)
  • Medium: DMEM (Nacalai tesque)
  • PlateGravityTRAP™ ULA plate (InSphero)
  • Seeding cell density: 500, 1000cells / well
  • Culture days: 5 days after spheroid formation by hanging drop
  • Imaging methods
    • Bright-field, 4800dpi
    • Bracket Focus (stacked)

Results and Conclusions

It was possible to quantify the spheroids formed by hanging drop for growth rate estimation.

             

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